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新型冠状病毒Spike S1-Fc蛋白 
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英文名称: Recombinant SARS-CoV-2 (2019-nCoV)  Spike Protein (S1, Fc Tag) 货号: XG100H1


SARS-CoV-2 Spike Protein S1, Fc fusion

(CHO-expressed full length S1-Fc fusion protein)


Accession #: GenBank: QIC53204.1.

Host: Chinese Hamster Ovarian cells

Protein: Recombinant full length SARS-CoV-2 Spike Protein (S1) protein

with human IgG1 fused at the C-terminus.

Purification: Protein A column.

Formulation: 1mg of purified S1-Fc protein in PBS. Protein

Concentration was determined by OD280 absorbance.

Physical State: Frozen liquid

Purity: > 85% based on SDS−PAGE

Storage: Store the product at -20℃. Product is stable for about 1 week at 2-8℃ as an undiluted liquid. Prepare working dilution fresh each day. Avoid repeated freezing and thawing.


FOR IN VITRO RESEARCH USE ONLY NOT FOR USE IN HUMANS


Research Applications

Indirect ELISA: for generation of neutralizing anti-SARS-CoV-2 antibodies.

Colloidal gold immunochromatographic: to be used as double antigen format.



Reference:

Zhao RQ, et al. Early detection of SARS-CoV-2 antibodies in COVID-19 patients as a serologic marker of infection, submitted. preprint:
https://www.medrxiv.org/content/10.1101/2020.03.17.20037713v1.full.pdf.


Ren WL, et al. “A COVID-19 vaccine based on the spike S1 protein with a novel adjuvant induces high level of neutralizing responses in nonhuman primates” submitted.



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